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. 2021 Apr 13;12:2198. doi: 10.1038/s41467-021-22522-4

Fig. 4. EFA6B knock-out promotes a change in the ITG repertoire and stimulates the formation of ITGβ1-based invadopodia.

Fig. 4

a Cell surface expression of ITG molecules in MCF10A WT (blue) and EFA6B KO55 (red) cells analyzed by FACS. b Representative images of the indicated spheroids grown 2 days in collagen I stained for the indicated ITG. Scale bars 20 μm. c Expression of ITGβ1 and ITGβ4 analyzed by immunoblot in MCF10A WT and EFA6B KO55 cells. HSP60 served as a loading control. N = 3. d Quantification of MCF10A WT and EFA6B KO55 cell aggregates with invasive protrusions incubated in the presence of the control pre-immune serum (Ctl) or the indicated anti-ITG (α-ITG) antibodies for 2 days. Values are percentages of total cell aggregates ± SEM. 300 cell aggregates were analyzed for each cell population in three independent experiments, one-way ANOVA test with Dunnett’s multiple comparison p-values. e Representative images of the indicated cells grown 2 days on collagen I-coated coverslips stained for ITGβ1 (green), MMP14-mCherry (red), and F-actin (blue). Co-localization of all three markers appears in white. The large inset is a 2× zoom-in image of the indicated area. Scale bars 20 μm. f Quantification of the percentage of ITGβ1 co-localized with MMP14-mCherry. A total of 45 WT cells and 42 KO55 cells from three independent experiments were analyzed, Student’s t-test p-value. Source data are provided as a Source Data file.