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. 2021 Mar 31;12:656353. doi: 10.3389/fmicb.2021.656353

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Copyright © 2021 Ullah, Sajid, Yan, Feng, He, Shereen, Li, Xu, Hao, Guo, Chen, Zhou and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IFI27 suppresses HBV replication and gene expression in vivo. (A–F) Two groups of C57BL/6 mice (n = 10) were co-injected with pHBV1.3 along with pCAGGS-HA-IFI27 or pCAGGS. After 4 days of hydrodynamic injection, the mice were sacrificed and the livers and blood were processed for analyses. (A) The expression level of IFI27 was determined by western blot using anti-HA antibodies, and the levels of β-actin serve as loading controls (lower panel). (B,C) The titer of secreted HBsAg (B) and HBeAg (C) proteins expressed in mice blood was measured using ELISA. (D) The level of HBV DNA from sera was also evaluated using qPCR. (E) Total HBV RNA was isolated from the liver tissues, and the levels of HBV pgRNA were measured by qRT-PCR. The level of mice GAPDH (mGAPDH) was used as an internal control. (F) Immunohistochemical staining was used to determine the HBcAg levels in the liver. **P ≤ 0.01, ****P ≤ 0.0001.