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. 2021 Apr 9;14:1341–1356. doi: 10.2147/JIR.S299927

Figure 1.

Figure 1

Rotenone dose-dependently induces LC/NE neurodegeneration and microglial activation in mice. (A) Mice were injected with the indicated dose of rotenone for 3 weeks. Immunohistochemistry with an anti-TH antibody was performed to stain NE neurons in the LC of mice, and representative images are presented. (B) Quantification of the number of THir neurons. (C) Immunohistochemistry with an anti-Iba-1 antibody was performed to stain microglial cells in the LC of mice, and representative images are presented. (D) Quantification of the density of Iba-1 immunostaining. Results were mean ± SEM from six mice for each group and were analyzed by one-way ANOVA (THir neurons counts: F(2,15) = 50.221, P = 0.000; Iba-1 density: F(2,15) = 8.158, P = 0.004; post hoc analysis by Tukey’s multiple comparisons test). **P<0.01; Scale bar = 100 μm.