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. 2021 Apr 9;14:1341–1356. doi: 10.2147/JIR.S299927

Figure 2.

Figure 2

Rotenone time-dependently induces microglial activation and LC/NE neurodegeneration in mice. (A) Microglial cells and LC/NE neurons from mice at the indicated time points after rotenone treatment were immunostained with anti-Iba-1 and anti-TH antibodies, respectively, and representative images are shown. (B) Quantification of the density of Iba-1 immunostaining. (C) Quantification of the number of THir neurons. Results were mean ± SEM from four mice for each group and were analyzed by one-way ANOVA (Iba-1 density: F(3,12) = 21.464, P = 0.000; THir neurons counts: F(3,12) = 8.849, P = 0.002; post hoc analysis by Tukey’s multiple comparisons test). (D) The expression levels of Iba-1 and TH in the brainstem of mice were determined by Western blotting with specific antibodies, and representative blots are shown. (E and F) Quantification of the band densities of Iba-1 (E) and TH (F). Results were mean ± SEM from four mice for each group and were analyzed by one-way ANOVA (Iba-1: F(3,12) = 6.744, P = 0.006; TH: F(3,12) = 20.506, P = 0.000; post hoc analysis by Tukey’s multiple comparisons test). *P<0.05, **P<0.01; Scale bar = 100 μm.