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. 2021 Mar 31;12:649463. doi: 10.3389/fimmu.2021.649463

Figure 6.

Figure 6

Tiliroside dampens glycolysis through enhancing HIF-1α proteasomal degradation. (A, B) The BMDMs were cultured with LPS (100 ng/ml) & IFNγ (20 ng/ml) and tiliroside (10, 20, 40 μM) for 24 h. The protein level of HIF-1α was analyzed by using western blotting assay (A); the mRNA expressions of Hif-1α were analyzed by using qPCR assay (B). (C) The BMDMs were treated with LPS (100 ng/ml) & IFNγ (20 ng/ml) for 24 h, and then treated with CHX (10 mg/ml) and tiliroside (40 μM) for the indicated periods. The protein level of HIF-1α was analyzed by using a western blotting assay. (D, E) The BMDMs were treated with LPS (100 ng/ml) & IFNγ (20 ng/ml) for 24 h, and then pretreated with MG132 (10 μM) or BafA1 (10 nM) for 2 h followed by incubation of tiliroside (40 μM). The protein level of HIF-1α was analyzed by using a western blotting assay. Data were expressed as means ± S.E.M of three independent assays. ## P < 0.01 vs. Control group.