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. 2021 Mar 31;15:649159. doi: 10.3389/fnins.2021.649159

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Copyright © 2021 Tsuneoka and Funato.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Galanin and neurotensin mRNA expression in the preoptic area. (A,B) Double ISH for (A) galanin and (B) neurotensin of a coronal section of the mouse brain (bregma + 0.10 mm). (C,D) Double ISH for (C) galanin and (D) neurotensin of a coronal section of the mouse brain (bregma –0.02 mm). (E,F) Double ISH for (E) galanin and (F) neurotensin of a coronal section of the mouse brain (bregma, –0.14 mm). (G,H) Double ISH for (G) galanin and (H) neurotensin of a coronal section of the mouse brain (bregma, –0.24 mm). Scale bars: 200 μm. 3v, third ventricle; ac, anterior commissure; ACN, anterior commissural nucleus; AH, anterior hypothalamus; BNST, bed nucleus of the stria terminalis; fx, fornix; LAN, lateroanterior hypothalamic nucleus LPOA, lateral preoptic area; MPOA, medial preoptic area; MPN, medial preoptic nucleus; NDB, nucleus of the diagonal band; opt, optic tract; PVPOA, periventricular preoptic area; SCN, suprachiasmatic nucleus; SI, substantia innominata; SON, supraoptic nucleus; VLPO, ventrolateral preoptic nucleus. Adapted from Tsuneoka et al. (2013).