Figure 6.

Absence of detectable neuroinflammatory reaction in the hippocampus of amplicon-injected animals

(A–H) Dorsal hippocampal, GFAP-stained sections prepared from a negative control, vehicle-injected (A), a positive control, JΔNI R0 injected (B), and various amplicon vector injected animals (C–H, as indicated), at 4 dpi. Note numerous GFAP-positive cells in the positive control, but not in other groups. (I–P) As above, IBA-1-stained. Note numerous IBA-1-positive cells in the positive control, but not in other groups. (Q–X) As above, CD45-stained. Again, note numerous CD45-positive cells in the positive control, but not in other groups. These images are representative of 5 section per animal, 6 animals per group except 4 animals per group in (C), (D), (K), (L), (S), and (T). Horizontal bar in (X) (for all panels), 70 μm.