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. 2021 Mar 31;12:640718. doi: 10.3389/fimmu.2021.640718

Figure 3.

Figure 3

Control and PH-fibroblast conditioned media have distinct effects on regulating macrophages metabolism. (A) Heat-map showing metabolites that are significantly regulated by PH-CM compared to CO-CM treated or untreated BMDMs (One-way ANOVA: p ≤ 0.05). (B) Metabolites in glycolytic or pentose phosphate pathway (PPP) that were significantly increased by PH-CM compared to untreated or CO-CM treated BMDMs. *p < 0.05, **p < 0.01. (C) Metabolites in glutaminolysis, TCA cycle, urea cycle or polyamine synthesis that were significantly increased by PH-CM compared to untreated or CO-CM treated BMDMs. *p < 0.05, **p < 0.01, ***p < 0.001. (D) CM was collected from CO-Fibs, PH-Fibs or PH-Fibs treated with 2DG. BMDM was exposed to CM for 18hrs prior to their analysis for extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) were measured with Seahorse XF96 analyzer to determine macrophage glycolysis and mitochondrial OXPHOS. ****p < 0.0001.