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. 2021 Mar 31;12:589940. doi: 10.3389/fpls.2021.589940

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Copyright © 2021 Makatsa, Tincho, Wendoh, Ismail, Nesamari, Pera, de Beer, David, Jugwanth, Gededzha, Mampeule, Sanne, Stevens, Scott, Blackburn, Mayne, Keeton and Burgers.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of plant-expressed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike antigens after Protein A purification. (A) Coomassie-stained SDS-PAGE gel and (B) Western blot of S1-rabbit Fc fusion protein (2 μg of concentrated elution fraction). Lines on the left indicate molecular weight marker (Spectra Multicolor Broad range protein ladder) in kDa. The arrow indicates the expected size for recombinant S1 protein (~140 kDa). (C) Coomassie-stained SDS-PAGE gel and (D) Western blot of RBD-rabbit Fc fusion protein (5 μg of concentrated elution fraction). Arrows indicate expected size for RBD-rabbit Fc conjugate (~100 kDa).