Table 1.

Defense and antioxidative response in coffee leaves sprayed with Calonectria hemileiae and inoculated with Hemileia vastatrix

Treatments	72 hbi					72 hai					50 dai
	CHI	GLU	APX	POX	SOD	CHI	GLU	APX	POX	SOD	CHI	GLU	APX	POX	SOD
Control	2.8b	3.1b	15.1a	0.10b	1.6a	5.2ab	5.7a	19.6a	0.2a	1.8ab	5.4ab	1.6b	26.8b	0.3ab	0.6b
Hv	–	–	–	–	–	5.0ab	4.7a	21.9a	0.2a	0.7b	7.3ab	5.0a	28.2b	0.2b	0.6b
Ch	12.7a	8.6a	22.2a	0.07b	1.5a	2.2c	1.4b	22.2a	0.2a	1.5ab	9.7a	2.3b	49.4a	0.4a	0.6b
Ch/72Hv	10.7a	5.4ab	29.1a	0.21a	0.8b	6.7a	2.6ab	24.4a	0.2a	2.1a	2.0b	0.9b	2.6c	0.2ab	1.4a
Ch/Hv	–	–	–	–	–	4.5abc	2.6ab	27.9a	0.3a	2.0a	5.1ab	2.3b	29.9b	0.2b	0.5b
Fungicide	–	–	–	–	–	2.5bc	2.7ab	24.1a	0.2a	1.6ab	4.1ab	0.4b	17.4bc	0.2ab	1.6a
F Values	26.8∗∗	4.56∗	2.43ns	33.6∗∗	15.4∗∗	6.95∗	4.52∗	1.47ns	1.53ns	3.76∗	3.66∗	10.9∗∗	17.1∗∗	3.69∗	8.90∗∗

Activities of chitinase (CHI, μmol min⁻¹ mg⁻¹ protein), β-1,3-glucanase (GLU, μmol min⁻¹ mg⁻¹ protein), ascorbate peroxidase (APX, μmol min⁻¹ mg⁻¹ protein), peroxidase (POX, μmol min⁻¹ mg⁻¹ protein), and superoxide dismutase (SOD, unit min⁻¹ mg⁻¹ protein) in the leaves of coffee plants that were treated with C. hemileiae at 72 h before as well as at 72 h and 50 days after inoculation with H. vastatrix and coffee plants that were treated with fungicides (trifloxystrobin + tebuconazol). Plants sprayed with sterile water and C. hemileiae without inoculation with H. vastatrix served as the control treatments. Treatment means followed by the same letter, within each experiment, are not significantly different (p ≤ 0.05) as determined by Tukey's test. ^{∗, ∗∗} = significant differences at 0.05 and 0.01, respectively. ^ns = not significant. n = 5.