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. 2021 Mar 31;11:650037. doi: 10.3389/fonc.2021.650037

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Copyright © 2021 Ren, Chen, Wang, Rui and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LINC00941 in NSCLC cells promoted angiogenesis of HUVECs. Stable LINC00941 knockdown A549 and NCI-1299 cells, and exogenously overexpressed LINC00941 in A549 and NCI-1299 cells were constructed. (A) The expression of LINC00941 was determined by qRT-PCR. (B) MTT assay used to detect proliferation of A549 and NCI-1299 cells. HUVECs were cultured with supernatant of A549 and NCI-1299 cells stably expressed LINC00941 or si-LINC00941. (C) MTT assay used to detect proliferation of HUVECs. (D) Effect of LINC00941 expression in NSCLC cells on HUVECs in tube formation assay. Scale bar, 100 μm. (E) Transwell assay was to examine invasion of HUVECs. Scale bar, 100 μm. (F) The expression of the proliferation and migration-related genes in HUVECs. (G) The level of VEFGA in NSCLC cells in ELISA assay. Data are mean ± SD; *P < 0.05. All experiments were repeated three times.