FIGURE 5.

Time- and concentration-dependent effects of ivermectin on proliferation and cell cycle. (A,B) Concentration-response curves upon 24–72 h ivermectin-treatment in MKN74 and KATO-III cells. Proliferation was assessed using CCK-8 Kit at 450 nm. IC₅₀ values were calculated from sigmoidal regression curve fitting using variable slope on normalized response from log₁₀-transformed x-values (GraphPad Prism v.6). (C) Cell cycle analysis of KATO-III cells. Means of n = 3 replicates/treatment with SD. One-way ANOVA with Dunnett’s post hoc test (2-sided) compared to respective control groups.