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. 2021 Mar 31;12:642274. doi: 10.3389/fphys.2021.642274

Figure 2.

Figure 2

Effect of apelin-13 on aquaporin 2 (AQP2) expression in the renal cortex and medulla in WD mice. (A,B) Representative immunoblotting and densitometric analysis of AQP2 protein expression in the kidney cortex (A) and medulla (B), the protein abundances were normalized by β-actin (C,D) qRT-PCR analysis of renal AQP2 mRNA expression in the kidney cortex (C) and medulla (D). The expression was normalized by GAPDH. (E,F) Representative immunoblotting and densitometric analysis of membrane and cytosolic fraction AQP2 protein expression in the kidney cortex (E) and medulla (F). The abundance of AQP2 in the membranous and cytoplasmic factions was normalized by α-NKA and β-actin, respectively. n = 5 per group. Data are mean ± SEM. *p < 0.05 and **p < 0.01 vs. Control; #p < 0.05 and ##p < 0.01 vs. WD.