Figure 4.

Effect of apelin-13 on intrarenal renin-angiotensin system (RAS) in the renal cortex and medulla in WD mice. (A,B) Representative immunoblotting and densitometric analysis of fPRR, soluble prorenin receptor (sPRR), renin, and ACE protein expression in the kidney cortex (A) and medulla (B), the protein abundances were normalized by β-actin. (C,D) qRT-PCR analysis of renal PRR, renin 1, and ACE mRNA expression in the kidney cortex (C) and medulla (D), expression was normalized by GAPDH. n = 4–5 per group. Data are mean ± SEM. ^*p < 0.05 and ^**p < 0.01 vs. Control; ^#p < 0.05 and ^##p < 0.01 vs. WD.