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. 2021 Mar 31;12:642274. doi: 10.3389/fphys.2021.642274

Figure 7.

Figure 7

Apelin regulates renal AQP2 expression via the cAMP/β-catenin/sPRR signaling pathway. (A,B) Representative immunoblotting and densitometric analysis of nuclear β-catenin protein expression in the renal cortex (A) and medulla (B) in WD mice. The expression of AQP2 protein was analyzed by immunoblotting and normalized by Histone-3 as an internal control for equal loading of the samples. n = 5 per group. Data are mean ± SEM. *p < 0.05 and **p < 0.01 vs. control; #p < 0.05 vs. WD. (C,D) Role of Wnt/β-catenin signaling pathway in response to 8-Br-cAMP in primary rat IMCD cells. Cells were pretreated with ICG001 and then treated with 100 μM 8-Br-cAMP for 24 h. (C) The expression of PRR and AQP2 protein was analyzed by immunoblotting and normalized by β-actin. (D) Medium sPRR levels were determined. n = 3 per group, repeated for three times. Data are mean ± SEM. *p < 0.05 and **p < 0.01 vs. Control; #p < 0.05 and ##p < 0.01 vs. 8-Br-cAMP. (E) Role of Wnt/β-catenin signaling pathway in response to AVP in primary rat IMCD cells. Cells were pretreated with ICG001 and then treated with 10 nM AVP for 24 h. The expression of PRR and AQP2 protein was analyzed by immunoblotting and normalized by β-actin. n = 3 per group, repeated for three times. Data are mean ± SEM. **p < 0.01 vs. Control; #p < 0.05 and ##p < 0.01 vs. AVP.