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. 2021 Mar 31;12:619932. doi: 10.3389/fimmu.2021.619932

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Copyright © 2021 Hui, Zhang, Zheng, Yang, Yu, An, Wei and Ren.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overall single-cell transcriptome characteristics of pTreg and iTreg cells. (A) The t-SNE projection of 3,273 pTreg and 3,205 iTreg cells, showing the formation of 8 main clusters. (B) PTreg and iTreg cells distribution in each clusters. (C) Heatmap showed the top 10 differential expressed genes of 8 clusters. (D,E) Functional enrichment analysis of pTreg and iTreg cells by metascape (Barplot). (F) Comparison of significantly differential expressed genes between pTreg and iTreg cells; (G) Pseudo-time development trajectories of Treg cells; (H) Changes of 35 Treg signature genes expression in pseudo-time trajectory.