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. 2021 Mar 31;12:619932. doi: 10.3389/fimmu.2021.619932

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Copyright © 2021 Hui, Zhang, Zheng, Yang, Yu, An, Wei and Ren.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Single-cell transcriprome characteristics of pTreg cells. (A) The UMAP distribution of 3,273 pTreg cells, showing 7 main clusters; (B) Pearson correlation coefficient analysis between each clusters; (C) Function enrichment analysis of Cluster 5 by metascape (Barplot); (D) Pseudo-time trajectory analysis of pTreg by Monocle 2, Treg cells mainly divided into three branches: activated, resting and prolifertative Treg; (E) Heatmap showed the top 5 marker genes of 7 clusters. (F) The expression changes of 35 Treg signature genes in pseudo-time trajectory.