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. 2021 Mar 31;12:619932. doi: 10.3389/fimmu.2021.619932

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Copyright © 2021 Hui, Zhang, Zheng, Yang, Yu, An, Wei and Ren.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Distinct TCR repertoires of pTreg and iTreg cells. (A) TCR clones supported by more than two cells in each cluster in pTreg; (B) TCR clones supported by more than two cells in each cluster in iTreg; (C) TCR similarity comparison between pTreg and iTreg by Morisita-Horn; (D) CFSE-labeled proliferation of iTreg and pTreg cells without Anti-CD3/anti-CD28 expander beads, IL-2, and rapamycin stimulation, and CFSE-labeled proliferation of pTreg with Anti-CD3/anti-CD28 expander beads, IL-2, and rapamycin stimulation; (E) TCR VDJC genes usage analysis of pTreg and iTreg cells.