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. 2021 Mar 31;12:651211. doi: 10.3389/fendo.2021.651211

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Copyright © 2021 Ehrlicher, Stierwalt, Newsom and Robinson

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Short-term high-fat feeding induced accumulation of mitophagy receptors. Autophagy protein markers in skeletal muscle of mice fed low-fat diet or high-fat diet for 3 days, 7 days, or 12 weeks. Quadriceps muscles were prepped as whole tissue lysates for immunoblotting of Beclin-1 (A). Gastrocnemius muscles were fractionated to prepare a mitochondrial-enriched fraction for immunoblotting of Parkin (B), Bnip3 (C), p62 (D), LC3II (E) and Lamp1 (F). Images below graphs are representative blot images. Protein targets in the mitochondrial fraction are normalized to Vdac abundance as a mitochondrial protein control. Panels (C–E) contain the same Vdac image because the targets are from the same membrane. Data are means ± SD; n=4-5. Two-way ANOVA was used to test for effects of diet and time. P values are main effects.