Table 3.
Manual methods used for PRP production from equine blood and potential classification according to the systems proposed for human PRP classification.
| Authors | Type of study | Design | Tubes and other materials used for PRP protocol | PLT, WBC, and RBC concentrations per μL of PRP | Human PRP Classification* |
|---|---|---|---|---|---|
| Fontenot et al. (66) | Hematological study | Comparison between two manual and semiautomated methods for producing PRP. | 60-mL syringe containing 8 mL of ACD-A. 10-mL plain glass tubes. Centrifugation at 1200 g/3 min Centrifugation at 2000 g/3 min. 50 mL conical tube |
PLT × 103/μL: 267.5 ± 142.8 WBC × 103/μL: ND RBC × 106/μL: NR |
It was no possible to classify these PRPs according to the published information. |
| PLT × 103/μL: 399.4 ± 157.1 WBC × 103/μL: ND RBC × 106/μL: NR |
|||||
| PLT × 103/μL: 433.0 ± 126.9 WBC × 103/μL: ND RBC × 106/μL: NR |
|||||
| Maciel et al. (70) | Experimental in vivo study | Evaluation of PRP in equine burns. | 15 mL Falcon tubes containing 10% sodium citrate. Centrifugation at 300 g/10 min | PLT × 103/μL: 723.0 ± 50 WBC × 103/μL: NR RBC × 106/μL: NR |
It was no possible to classify this PRP according to the published information. |
| Giraldo et al. (19) | Hematological study | Evaluation of the effect of intrinsic factors on equine PRP. n = 40 | 8.5-mL ACD-A tubes that were centrifuged two times at 120 g/5 min and 240 g/5 min. | PLT × 103/μL: 304.3 ± 43.9 WBC × 103/μL: 4.3 ± 2.2 RBC × 106/μL: 0.14 ± 0.1 |
P-PRP (61) P2B (60) Type 1B (64) |
| Hessel et al. (68) | Hematological study | Were compared hematological variables of equine PRPs obtained with 4 commercially available systems and a non-commercial double-centrifugation technique. n = 6 horses. | 60-mL syringe containing 8 mL of ACD-A. 10-mL plain glass tubesCentrifugated two times at 300 g/10 min. |
PLT × 103/μL: 310.4 ± 164.5 WBC × 103/μL: 18.2 ± 11.8 RBC × 106/μL: NR |
L-PRP (61) P2A (60) Type 1B (64) |
| Moraes et al. (71) | Experimental in vivo research | Evaluation of the effect of PRP in equine joints. n = 8 horses. | Tubes containing sodium citrate that were centrifugated two times at 150 g/5 min and 800 g/5 min. | PLT × 103/μL: 423.0 ± NR WBC × 103/μL: 8.36 ± NR RBC × 106/μL: NR |
L-PRP (61) P2A (60) Type 1B (64) |
*
For acronym mean, please see Table 1.