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. 2021 Mar 31;12:643951. doi: 10.3389/fmicb.2021.643951

FIGURE 7.

FIGURE 7

Macrophage infection with M. smegmatis RmlA mutants. (A) Survival of wild type and mutants of RmlA in primary human monocyte–derived macrophage THP-1 with a series of time points. The experiment was performed in triplicates (n = 3). Error bars indicate standard error of mean. Asterisk indicates significant difference (P < 0.05). (B) Quantitative PCR analysis of TNF-α mRNA level in THP-1 macrophages infected for 12–48 h with wild-type M. smegmatis or RmlA mutant M. smegmatis. (C) ELISA analysis of TNF-α expression in supernatants of THP-1 macrophages for 12–48 h with wild-type M. smegmatis or RmlA mutant M. smegmatis. (D) Quantitative PCR analysis of IL-1β mRNA in THP-1 macrophages infected for 12–48 h with wild-type M. smegmatis or RmlA mutant M. smegmatis. (E) ELISA analysis of IL-1β expression in supernatants of THP-1 macrophages for 12–48 h with wild-type M. smegmatis or RmlA mutant M. smegmatis.