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. 2021 Apr 12;49(4):03000605211004024. doi: 10.1177/03000605211004024

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© The Author(s) 2021

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Verification of bone formation after 21 days in culture. For all panels, the 50:50 MSC/EPC group was used as control. (a) DNA content in each group at day 21. (b) Alkaline phosphatase (ALP) activity in each group at day 21. ALP activity and mineralization (evaluated by calcium content) were normalized to DNA content. ALP activity was increased in the 50:50 MSC/HSC and 50:25:25 MSC/HSC/EPC groups compared with the control group, but the differences were not significant. (c) Mineralization in each group at day 21. Mineralization was increased in the 50:50 MSC/EPC group and slightly decreased in the 50:25:25 MSC/HSC/EPC group. For all panels, values given as mean and standard deviation (error bars) (n = 6). *P < 0.05.

MSC, mesenchymal stem cell; EPC, endothelial progenitor cell; HSC, hematopoietic stem cell.