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. 2021 Feb 11;62:100048. doi: 10.1016/j.jlr.2021.100048

Fig. 1.

Fig. 1

Adrenal glands and lipid droplets are enlarged in Plin2−/− mice. Plin2+/+ and Plin2−/− mice were housed with ad libitum access to chow diet (Fed) or were fasted for 24 h (Fast) prior to euthanasia at 8–10 AM. Adrenal glands were dissected out, and the surrounding fat capsule was removed prior to analysis. A: Representative pictures of adrenal glands (pairs) from 30-week-old female Plin2+/+ and Plin2−/− mice. B: Adrenal weights of 15-week-old males (n = 6–9), 15-week-old females (n = 9–10), and 30-week-old females (n = 8–9) presented as means ± SD. Weights are presented as the average weight of the adrenal pair dissected from each mouse. C: Representative pictures of adrenals from fed and fasted 15-week-old female Plin2+/+ and Plin2−/− mice. Cryosections (20 μm thick) were stained to visualize LDs (Bodipy 493/503, green), nuclei (Hoechst 33342, blue), and plasma membrane-located F-actin (Phalloidin-CF568, red). I: Whole section scans at the center planes of adrenals. II: Confocal images of the central layer of the adrenal cortex (zona fasciculata) taken with a 40× objective. III: Pictures zoomed in from II to visualize individual LDs. Scale bars: 500, 20, and 10 μm. D: Quantification of average LD size, LD number per cell, and cortical cell size based on confocal images. Results are presented as means ± SD (n = 5–6, with 10 images analyzed for each animal). ∗∗∗P < 0.001 indicates difference between Plin2+/+ and Plin2−/− mice; #P < 0.05, ##P < 0.01 indicates difference between fed and fasted mice of the same genotype. LD, lipid droplet.