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. 2021 Feb 11;62:100048. doi: 10.1016/j.jlr.2021.100048

Fig. 4.

Fig. 4

Serum corticosterone levels are unaltered in young Plin2+/+ and Plin2−/− mice. Male and female 15-week-old Plin2+/+ and Plin2−/− mice with ad libitum access to chow diet (Fed) or fasted for 24 h (Fast) were euthanized at 8–10 AM or subjected to ACTH stimulation. A: Expression of mRNAs for key enzymes in corticosterone synthesis (Star and Cyp11a1) relative to the expression of Tbp in fed or fasted female mice (n = 7–8). Results are presented as means ± SD (∗P < 0.05 indicates difference between Plin2+/+ and Plin2−/− mice, ##P < 0.01 indicates difference between fed and fasted mice). B: Serum corticosterone levels in fed or fasted male and female Plin2+/+ and Plin2−/− mice at 8–10 AM (n = 8). Results are presented as means ± SD (##P < 0.01 indicates difference between fed and fasted mice of the same genotype). C: Serum corticosterone levels in male and female Plin2+/+ and Plin2−/− mice after ACTH stimulation. ACTH (i.p., 0.1 μg/g body weight) was administered to ad libitum fed mice at 9 AM. Blood samples collected before ATCH injection (baseline) and 1 and 2 h after injection were used to measure serum corticosterone. Results are presented as means ± SEM (males: n = 4; females: n = 6; ##P < 0.01 indicate differences from baseline). ACTH, adrenocorticotropic hormone; Cyp11a1, cytochrome P450 family 11 subfamily A member 1; Star, steroidogenic acute regulatory protein; Tbp, TATA-box binding protein.