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. 2021 Feb 11;62:100048. doi: 10.1016/j.jlr.2021.100048

Fig. 6.

Fig. 6

Accumulation of ceroid-like structures in adrenal glands is sex and age dependent. Adrenals were collected from 5-, 15-, and 50-week-old female and male Plin2+/+ and Plin2−/− mice with ad libitum access to chow and used for histological or lipid analyses. A: Representative images of adrenals of female and male Plin2+/+ and Plin2−/− mice sectioned through the center of the organ (n ≥ 3). Sections were stained with Hoechst 33342 to visualize nuclei (blue), Bodipy 493/503 to visualize lipid droplet (green), and CF568-Phalloidin to visualize plasma membranes (F-actin, red). The autoflourescent ceroid-like aggregates appear as orange/red structures. B: Phospholipids (PC, PS, and PE) in adrenals of 50-week-old female Plin2+/+ and Plin2−/− mice analyzed with TLC. Extracted lipids normalized to 100 μg adrenal weight were applied per lane. PC-34:1, PS-34:1, and PE-34:1 were used as phospholipid standards. Following development of neutral lipids (not shown), a second developing phase was applied to separate polar phospholipids. The arrows indicate unidentified non/slow-migrating (highly polar) lipids present in Plin2−/− but hardly visible in Plin2+/+ adrenals (n = 3). C: Lysophosphatidylcholine (LPC) species in adrenals of 50-week-old female Plin2+/+ and Plin2−/− mice analyzed with HPLC-qTOF/MS. D: Phosphatidylglycerol (PG) species in adrenals of 50-week-old female Plin2+/+ and Plin2−/− mice analyzed with HPLC-qTOF/MS. Results are presented as means ± SD (n = 3) relative to tissue weight. LPC, lysophosphatidylcholine; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PS, phosphatidylserine.