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. 2021 Feb 11;62:100048. doi: 10.1016/j.jlr.2021.100048

Fig. 7.

Fig. 7

Expression of lysosomal, lipophagy, and cholesterol efflux-related genes in adrenals of Plin2+/+ and Plin2−/− mice. Fifteen-week-old female Plin2+/+ and Plin2−/− mice with ad libitum access to chow (Fed) or after 24 h of fasting (Fast) were euthanized at 8–10 AM. Adrenals were dissected free from the surrounding fat capsule prior to analyses. A: Expression of mRNAs encoding proteins associated with lysosomal lipid droplet degradation (Lipa), lysosomal cholesterol transport (Npc1 and Npc2), and lysosomal membranes (Lamp1 and Lamp2) relative to the expression of Tbp (n = 7–8). B: Protein content of cytosolic LC3 (LC3-I) and lipidated LC3 (LC3-II), lysosomal membrane proteins (Lamp1 and Lamp2), and the autophagy receptor (p62) in Plin2+/+ and Plin2−/− adrenals. C: Quantification of immunoblot signals in B relative to levels measured for fed Plin2+/+ adrenals (n = 3). D: Expression of mRNAs encoding key transcription factors (Nr1h2, Nr1h3, and Srebf1 isoform c) and transporters related to efflux of cholesterol (Abca1, Abcg1, ApoE) relative to the expression of Tbp (n = 7–8). E: Expression of genes related to macrophage infiltration and inflammation (Adgre1, Tnf, and Il6) relative to the expression of Tbp (n = 7–8). Gene expression data and quantified immunoblots are presented as means ± SD (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 indicate difference between Plin2+/+ and Plin2−/− mice; #P < 0.05, ##P < 0.01, ###P < 0.001 indicate difference between fed and fasted mice of the same genotype). Abca1, ATP-binding cassette, subfamily A, member 1; Abcg1, ATP-binding cassette, subfamily G, member 1; Adgre1(F4/80, macrophage marker), adhesion G protein-coupled receptor E1; ApoE, apolipoprotein E; Lamp, lysosome-associated membrane protein; LC3, microtubule-associated protein 1A/1B-light chain 3; Lipa, lysosomal acid lipase A (LAL); Npc1/2, NPC intracellular cholesterol transporter 1/2; Nr1h2, nuclear receptor subfamily 1 group H member 2 (LXRβ); Nr1h3, nuclear receptor subfamily 1 group H member 3 (LXRα); p62, ubiquitin-binding protein p62; Srebf1c, sterol regulatory element binding transcription factor 1 isoform c (SREBP-1C); Tbp, TATA-box binding protein.