Reagent	Final concentration	Amount
Tris base	50 mM	0.30 g
Potassium glutamate	100 mM	0.93 g
1 M magnesium acetate	10 mM	0.5 mL
1 M DTT	5 mM	250 μL
Tween 20	0.01%	5 μL
Glycerol	5% v/v	2.5 mL
Total	n/a	50 mL

Adjust pH to 7.5 with acetic acid at 25°C. The buffer can be stored at 4°C for a few days.