Reagent	Final concentration	Amount
Sodium phosphate, dibasic	50 mM	0.36 g
NaCl	300 mM	0.88 g
glycerol	10% v/v	5 mL
Roche-Complete Protease Inhibitor Mixture Tablet	n/a	1 tablet
PMSF in methanol (50 mM)	0.1 mM	0.1 mL
Total	n/a	50 mL

Adjust pH to 8 with phosphoric acid at 25°C. The buffer can be stored at 4°C for a few weeks.