Figure 1.

Examples of how samples look before and after different steps of the adipose tissue digestion protocol

(A) Ensure adipose tissue samples are thoroughly washed and processed to remove residual blood and non-adipose tissue (e.g., connective tissue, cauterized tissue).

(B) If there are still lumps of adipose tissue visible within the sample (Left) after step 3 of the digestion protocol, then return samples to the shaker-incubator until they form a homogeneous mixture (Right).

(C) If samples look partially digested (Left) after step 5, with adipose tissue still visible, then carry out the additional re-digestion step before proceeding to step 6. If samples look completely digested (Right), with only lipid droplets visible, then proceed to step 6 of the adipose tissue digestion protocol.