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. 2021 Mar 30;2(2):100422. doi: 10.1016/j.xpro.2021.100422

Figure 4.

Figure 4

Samples contaminated with different non-adipose tissues can affect experimental data

(A) Tissue samples that sink in Wash/Digestion buffer are most likely not adipose tissue. When acquiring adipose tissue (AT) from mice, if testes (middle) or lymph nodes (LN) (right) are accidentally acquired, ensure they are removed before digestion.

(B) Tissue contaminants can distort different cell populations. As shown via flow cytometry analysis, when lymph nodes from a mouse are present during digestion, samples have increased CD45+ cells. This can compromise studies investigating T cell (CD45+ CD3+) abundance (right).