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Collagenase II
| Reagent | Final Concentration (mM or μM) | Amount |
|---|---|---|
| Collagenase from Clostridium Histolyticum (Type II) | 14,220 U/mL | Varies by batch |
| TES | 50 mM | 572.5 mg |
| CaCl2 | 360 μM | 2.645 mg |
| ddH2O | n/a | 50 mL |
| Total | n/a | 50 mL |
Store at −20°C for up to 1 year. Use 1M NaOH to adjust to pH of 7.4.
