MACS Buffer

Reagent	Final Concentration (mM or μM)	Amount
Fetal Bovine Serum	2%	10 mL
EDTA (0.5 M)	2 mM	2 mL
Dulbecco's Phosphate Buffered Saline pH 7.2 (without CaCl2 and MgCl2)	1×	488 mL
Total	n/a	500 mL

Store at 4°C for up to 3 months.