REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-human CCR7	BioLegend	Cat# 353216; RRID: AB_10916386
Anti-human CD19	BioLegend	Cat# 302228; RRID: AB_893272
Anti-human CD45	BioLegend	Cat# 304048; RRID: AB_2563129
Anti-human CD3	BioLegend	Cat# 300318;RRID: AB_314054
Anti-human CD4	BioLegend	Cat# 357410; RRID: AB_2565662
Anti-human CD8α	BioLegend	Cat# 300920; RRID: AB_528885
Anti-human CD45RO	BioLegend	Cat# 304224; RRID: AB_2563817
Anti-human CD14	BioLegend	Cat# 325608; RRID: AB_830681
Anti-human CD16	BioLegend	Cat# 302026; RRID: AB_2278418
Anti-human CD206	BioLegend	Cat# 321126; RRID: AB_2563839
Anti-human CD141	BioLegend	Cat# 344110; RRID: AB_2561623
Anti-human Clec9A	BioLegend	Cat# 353804; RRID: AB_10965546
Anti-human CD1c	BioLegend	Cat# 331520; RRID: AB_10644008
Anti-mouse CD45	BioLegend	Cat# 103146; RRID: AB_2564003
Anti-mouse CD11c	BioLegend	Cat# 117317; RRID: AB_493569
Anti-mouse CD11b	Thermo Fisher Scientific	Cat# 47-0112-82; RRID: AB_1603193
Anti-mouse MHCII	BioLegend	Cat# 107622; RRID: AB_493727
Anti-mouse CD8α	Thermo Fisher Scientific	Cat# 17-0081-82; RRID: AB_469335
Chemicals, peptides, and recombinant proteins
Deoxyribonuclease I from bovine pancreas	Sigma	Cat# D4527
Collagenase from Clostridium histolyticum (type II)	Sigma	Cat# C6885
Fetal bovine serum	SeraLab	Cat# EU-000-F
Dulbecco's phosphate buffered saline pH 7.2 (without CaCl2 and MgCl2)	Sigma	Cat# D8537-500ML
EDTA solution	Sigma	Cat# E7889-100ML
NH4Cl	Sigma	Cat# A9434-1KG
KHCO3	Sigma	Cat# 237205-100g
Na3EDTA	Sigma	Cat# ED3SS-1KG
TES	Sigma	Cat# T1375-100G
CaCl2	Sigma	Cat# C7902-500G
NaCl	Sigma	Cat# S7653-1KG
Critical commercial assays
Dead Cell Removal Kit	Miltenyi Biotec	Cat# 130-090-101
CD11c MicroBeads UltraPure, mouse	Miltenyi Biotec	Cat# 130-125-835
Other
7 mL Bijou container	Thermo Fisher Scientific	Cat# 129A
50 mL Falcon tubes	Corning	Cat# 430829
100 μm Cell strainer	Fisher	Cat# 11517532
70 μm Cell strainer	Greiner	Cat# 542070
V-Bottom 96-well plate	Sarstedt	Cat# 83.3926.500
LIVE/DEAD Fixable Aqua Stain	Invitrogen	Cat# L34966
Shaker-incubator (225 rpm, 37°C)	N/A	N/A
Refrigerated centrifuge (800 ×g, 4°C)	N/A	N/A