Table 1.
rRNA depletion oligos for S. cerevisiae
| Oligo Name | Sequence 5′ to 3′ |
|---|---|
| 5.8S-1 | TATTCCAGGGGGCATGCCTGTTTGAGCGTCATT |
| 5S-1 | ACCATACGCGAAACTCAGGTGCTGCAATCT |
| 15S-1 | GTAAAAAATTTATAAGAATATGATGTTGGTTCA |
| 15S-2 | TCAGATTAAGCGCTAAATAAGGACATGACACAT |
| 18S-1 | GTTT GACCTCAAATCAGGTAGGAGTACCCGCTGAACT |
| 18S-2 | AAACTTTCAACAACGGATCTCTTGGTTCTCG |
| 18S-3 | TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTC |
| 18S-4 | CAAAGATTAAGCCATGCATGTCTAAGTATAAGC |
| 18S-5 | CAGTGAAACTGCGAATGGCTCATTAAATCAG |
| 18S-6 | CATGGTATAACTGTGGTAATTCTAGAGCTAATACATGC |
| 18S-7 | ACCCTTTGGAAGAGATGTATTTATTAGAT |
| 18S-8 | TGATGATTCATAATAACTTTTCGAATCGCATGGC |
| 18S-9 | TGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTT |
| 18S-10 | TCCTAATTCAGGGAGGTAGTGACAATAAATAACGA |
| 18S-11 | TACCTTAACGAGGAACAATTGGAGGGCAAGTC |
| 18S-12 | AGCAGCCGCGGTAATTCCAGCTCCAATAGC |
| 18S-13 | CGTGTACTGGATTTCCAACGGGGCCTTT |
| 18S-14 | TTGAAAAAATTAGAGTGTTCAAAGCAGGCGT |
| 18S-15 | TTGCTCGAATATATTAGCATGGAATAATAGAAT |
| 18S-16 | GTAATGATTAATAGGGACGGTCGGGGGCAT |
| 18S-17 | TGCCAAGGACGTTTTCATTAATCAAGAACGA |
| 18S-18 | ACTATGCCGACTAGGGATCGGGTGGTGTT |
| 18S-19 | CTCGGCACCTTACGAGAAATCAAAGTC |
| 18S-20 | AGGTCCAGACACAATAAGGATTGACAGATTGA |
| 18S-21 | AGTTGGTGGAGTGATTTGTCTGCTTAATTGC |
| 18S-22 | CGCTACACTGACGGAGCCAGCGAGTCTAACC |
| 25S-1 | GACCTCAAATCAGGTAGGAGTACCCGCTGAACT |
| 25S-2 | GGAAAAGAAACCAACCGGGATTGCCTTAG |
| 25S-3 | CTTGGAACAGGACGTCATAGAGGGTGAGAATC |
| 25S-4 | AGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGT |
| 25S-5 | CAGTGATGGAAAGATGAAAAGAACTTTGAAAAG |
| 25S-6 | TCTAACGTCTATGCGAGTGTTTGGGTGTAAAAC |
| 25S-7 | CAAGAGGTGCACAATCGACCGATCCTGATGT |
| 25S-8 | ATCGAACCATCTAGTAGCTGGTTCCTGCCGAAGT |
| 25S-9 | ACGTAGAGTTAAGGTGCCGGAATACACGCTC |
| 25S-10 | ATATGGATTCTTCACGGTAACGTAACTGAATG |
| 25S-12 | TCCACAGGAAGGAATAGTTTTCATGCCAGGTCGTAC |
| 25S-13 | TCAAAGTGAAGAAATTCAACCAAGCGCGGGTAA |
| 25S-14 | GAGGGTGTAGAATAAGTGGGAGCTTCGGCGC |
| 25S-15 | ATAGGGAACGTGAGCTGGGTTTAGACCGTCG |
| 25S-16 | CTCTTCCTATCATACCGAAGCAGAATTCGG |
| 25S-17 | ACACAATATAGATGGATACGAATAAGGCGTC |
| 25S-18 | TACTGATGAATGTTACCGCAATAGTAATTG |
| 25S-19 | GAGGAACAGTTCATTCGGATAA |
| 25S-20 | AATCATTTGTATACGACTTAGATGTACAACGGG |
| 21S-1 | GTAAAAAGTAGAATAATAGATTTGAAATA |
| 21S-2 | TAGATTTAAAGAGATAATCATGGAGTATAA |
| 21S-3 | TATAAACTAATAAAGATCAGG |
| 21S-4 | CCGTAATGTAGACCGACTCAGGTATGTAAGTA |
The mixed rRNA depletion oligos used in Step 27. The stock concentration is 200 μM. Working concentration is 2 μM.