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. 2021 Mar 31;2(2):100447. doi: 10.1016/j.xpro.2021.100447
Reagent Final Concentration Amount
Total ligated RNA 10.6 μL
5PSeq RT oligo (20 μM) 1 μM 1 μL
5PSeq dT oligo (0.05 μM) 0.5 nM 0.2 μL
dNTPs (10 mM) 0.05 mM 0.1 μL
Total 12.8 μL