Reagent | Final Concentration | Amount |
---|---|---|
First-strand buffer (5×) | 1× | 4 μL |
DTT (100 mM) | 10 mM | 2 μL |
Ribolock (40 U/μL) | 0.4 U/μL | 0.2 μL |
Total | 6.2 μL |
Reagent | Final Concentration | Amount |
---|---|---|
First-strand buffer (5×) | 1× | 4 μL |
DTT (100 mM) | 10 mM | 2 μL |
Ribolock (40 U/μL) | 0.4 U/μL | 0.2 μL |
Total | 6.2 μL |