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. 2021 Mar 31;2(2):100447. doi: 10.1016/j.xpro.2021.100447
Reagent Final Concentration Amount
First-strand buffer (5×) 1× 4 μL
DTT (100 mM) 10 mM 2 μL
Ribolock (40 U/μL) 0.4 U/μL 0.2 μL
Total 6.2 μL