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. 2021 Mar 31;2(2):100447. doi: 10.1016/j.xpro.2021.100447
PCR Cycling Conditions
Steps Temperature Time Cycles
Initial Denaturation 98°C 30 sec 1
Denaturation 98°C 20 sec 14–18 cycles
Annealing 65°C 30 sec
Extension 72°C 30 sec
Final extension 72°C 7 min 1
Hold 4°C