REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
DMEM/F12	Gibco	31330-038
L-Glutamine	Thermo Scientific	25030-024
Penicillin-streptomycin (Pen/Strep)	Gibco	15140-122
A83-01 (TGFβ pathway inhibitor)	Sigma-Aldrich	SML0788
Nicotinamide	Sigma-Aldrich	N0636
N2	Gibco	17502-048
B27 minus vitamin A	Gibco	12587-010
N-Acetylcysteine	Sigma-Aldrich	A7250
17-β Estradiol	Sigma-Aldrich	E2758
SB203580 (p38 MAP kinase inhibitor)	Biotechne (Tocris)	1264
Human epidermal growth factor (EGF)	R&D Systems	236-EG
Human noggin	PeproTech	120-10C
Human R-spondin 1 (RSPO1)	PeproTech	120-38
Human insulin-like growth factor-I (IGF1)	PeproTech	100-11
Human hepatocyte growth factor (HGF)	PeproTech	100-39
Human Neuregulin-1 (NRG1)	PeproTech	100-03
InSolution Y-27632 (ROCK Inhibitor, RI)	Sigma-Aldrich	688001
Fetal bovine serum (FBS)	Sigma-Aldrich	F7524
Dimethylsulfoxide (DMSO)	Sigma-Aldrich	D2650
Phosphate buffered saline (PBS)	Gibco	10010-015
Collagenase IV	Gibco	17104-019
Matrigel (growth factor-reduced; phenol red-free)	Corning	356231
TrypLE express	Gibco	12605-010
Paraformaldehyde (PFA), 16%	Merck	8.18715
DNase	Sigma-Aldrich	D5025
Ethanol absolute, ≥99.8% (EtOH)	Fisher Chemical	E/0650DF/15
N-Butanol	Honeywell	33065
XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide)	Invitrogen	X6493
PMS (N-methyl dibenzopyrazine methyl sulfate)	J&K Scientific	276473
Biological samples
Biopsies derived from patients with ovarian cancer	N/A	N/A
Software and algorithms
GraphPad Prism V.8.0.1	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
Other
48-well flat bottom plates	Corning	3548
96-well flat bottom plates	Thermo Scientific	167008
Steriflip-GP Sterile Centrifuge Tube Top Filter Unit, 0.22 μm	Millipore	SCGP00525
Cryovials	Thermo Fisher Scientific	375353
Flat-bottom tubes of glass	Karl Hecht	42779065
1.5 mL Eppendorf tube	Eppendorf	0030120.086
15 mL Centrifuge tube	Corning	430052
50 mL Conical tube	Corning	430290
30 mL Conical tube	Fisher Scientific	11339133
Sterile 10 μL pipette tips with filter	Greiner	771288
Sterile 20 μL pipette tips with filter	Greiner	774288
Sterile 200 μL pipette tips with and without filter	Greiner	739288
Sterile 1000 μL pipette tips with filter	Greiner	740288
Pipette (P10, P20, P200, P1000)	Eppendorf or others	2231300006
Scalpel (surgical blade)	Swann-Morton	0207
Petri dish	Corning	353002
Plastic transfer pipette (3.5 mL)	Sarstedt	86.1171.001
Block heater	VWR	460-2116
70 μm Cell strainer	Corning (Falcon)	352350
Stainless steel base molds	Simport	M474-2
Millex-GV Syringe Filter Unit, 0.22 μm	Millipore	SLGV033R
Cryobox (Mr. Frosty Freezing Container)	Thermo Scientific	5100-0001
37°C Water bath	Thermo Fisher	15187015
Shaking plate	VWR Collection	444-2900
37°C and 5% CO2 tissue culture incubator	Thermo Fisher	51026282
Inverted microscope (such as Axiovert 40 CFL Trinocular)	Zeiss	N/A
Bunsen burner	N/A	N/A
Biological safety cabinets	Esco	AC2-6S8
Chemical hood	N/A	N/A
HistoStar Embedding Workstation	Thermo Scientific	A81000001
Cartridge burner	Usbeck	1420
Microplate reader VICTOR	PerkinElmer	2030-0050