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. 2021 Mar 29;9:633358. doi: 10.3389/fcell.2021.633358

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Copyright © 2021 Dong, Dong, Yang, Zhu, Gao, Chen, Zhao and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of exosomes derived from liver cancer cells. (A) Transmission electron microscopy analysis of exosomes (Exos) derived from HCC cell lines (HCCLM3, MHCC97L, and PLC/RFP/5). Scale bar, 200 nm. (B) Immunoblotting assay of exosomal biomarkers in different liver cancer cells. (C) Mean diameter and size distribution of HCC-Exos was detected by Nanoparticle tracking analysis. (D) Strategy for exosomes purifcation from the HCC cell lines culture supernatants based on differential centrifugations.