Figure 2.

Sequence alignment of LmaCK1.2 (A) and PfCK1 (B) with human CK1δ TV1. (A) Protein sequences of the major casein kinase 1 (CK1) isoform of Leishmania (LmaCK1.2) and human CK1δ TV1 were aligned using ClustalW (Thompson et al., 1994). Full conservation of amino acids (*), strong conservation (:) and weak conservation (.) is indicated in the line between the sequences. Secondary structure features were predicted using CFSSP (Ashok Kumar, 2013) and are indicated by a color code next to the respective sequence. Specific motifs, residues involved in ATP-binding (red characters), as well as residues involved in substrate recognition (green characters) for human CK1δ are indicated in the figure as well as the alternative residue in LmaCK1.2, if different. (B) Protein sequences of PfCK1 of Leishmania (LmaCK1.2) and human CK1δ TV1 were aligned as described in (A).