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. 2021 Mar 31;12:576340. doi: 10.3389/fpls.2021.576340

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Copyright © 2021 Lian, Zhang, Huang, Huang, Zhang and Hu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Syntenic and tissue expression of OlSSs and OlSP. (A) Syntenic representation of OlSSs and OlSP in Oryza sativa and Oryza longistaminata. Os05g0197900, which encodes an unknown DUF247 domain protein in O. sativa, corresponds to OlSS1, whereas Os05g0198000 and Os05g0198100 correspond to OlSP and OlSS2 respectively. (B) Analysis of OlSS1, OlSS2, and OlSP protein domains. Both OlSS1 and OlSS2 contain a conserved DUF247 domain, whereas OlSP contains an N-terminal YfaZ domain of unknown function. (C) RT-PCR determination of the tissue-specific expression pattern of OlSSs and OlSP in O. longistaminata. OlSS1-1 and OlSS1-2 are mainly expressed in stamens, whereas OlSS2-1 and OlSS2-2 are expressed in both stamens and pistils, and OlSP-1 and OlSP-2 are highly and specifically expressed in pistils. OlSS1: Self-incompatibility stamen1 from O. longistaminata; OlSS2: Self-incompatibility stamen2 from O. longistaminata; OlSP: Self-Incompatibility Pistil factor from O. longistaminata; O. sativa A05: Oryza sativa chromosome 5.