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Expression of HIF-1α and its downstream proteins in irradiated hepatoma cells. Proteins were obtained from the two cell lines of HepG2 cells (A,B) and T98G cells (C,D) at 0, 2, 8, and 24 h post 5 Gy X-ray irradiation. The expression level of HIF-1α and its downstream proteins was compared with the corresponding β-actin and then normalized to that protein from cells under normoxia conditions. a, p < 0.05, compared with the normoxic control in the absence of irradiation.
