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. 2021 Mar 25;9:637454. doi: 10.3389/fcell.2021.637454

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Copyright © 2021 Zhang, Zhang, Mo, Patel, Butterworth, Shao and Prise.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expressions of HIF-1α and downstream proteins in hypoxic-irradiated cells pretreated with HIF-1α inhibitor treatment. The HepG2 cells (A,C) and T98G cells (B,D) were pretreated with or without 100 μM CoCl₂, 5 μM 7-aminoflavone for 4 h, and HIF-1α siRNA for 24 h before irradiation under normoxic or hypoxic conditions, then nuclear or whole proteins were collected and analyzed at 4 h post 5 Gy X-irradiation. The HIF-1α signal and its downstream proteins were compared with the corresponding β-actin and then normalized to that protein under hypoxia. a, p < 0.05 compared with the normoxic control; b, p < 0.05 compared with the hypoxic control without irradiation.