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. 2021 Mar 29;12:653344. doi: 10.3389/fimmu.2021.653344

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Copyright © 2021 Chen, Han, Chen, Xie, Yang, Zhou, Zhang and Xia

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification and expression of inflammation-related cytokines in neutrophils in vitro. RT-qPCR (A–D) and ELISA (E–H) detection in the supernatant of IL-6, IL-8, TNF-α, and IL-10 in human freshly isolated neutrophils. Cells were incubated with miR-let-7b mimics, miR-let-7b inhibitor, and miR-let-7b mimics NC (100 nM, respectively), and the supernatant was collected at 10 hours after stimulation with 100 ng/mL LPS for 4 h. Data represent mean ± SD (n=3–5). Tukey’s multiple comparison tests were used to generate the P-values indicated in the figure. *P < 0.05, **P < 0.01, ***P < 0.001.