Figure 3.

GSK126 attenuates the activation of IFN-I signaling pathway. (A, B) THP-1 cells were pretreated with different concentrations of GSK126 for 30 min, then stimulated with universal type I IFN (1,000 U/ml) for 4 h. The levels of CXCL10 mRNA (A) and IFIT3 mRNA (B) were measured. (C, D) IFN-I induced phosphorylation of STAT1 was determined in THP-1 cells pretreated with 5 μM of GSK126 by Western bolt. (C) Representative pictures of at least three independent experiments. (D) Band densities quantified by Image J (A, B, D) Data are presented as mean ± SEM. (A, B) P values were determined by Mann-Whitney U-test. *P < 0.05, ns, not significant. (D) P value was determined by 2-way ANOVA.