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. 2021 Mar 23;12:642678. doi: 10.3389/fimmu.2021.642678

Figure 6.

Figure 6

Percentage of histone H3 modification in total H3 in Tregs and Teffs. (A) Comparison of percentage of modifications in Tregs: POLY, SPEC, UNSPEC and Teffs: POLY, SPEC, UNSPEC. (B) Comparison of modifications only in Tregs. Cells were stimulated with anti-CD3/anti-CD28 beads (index POLY) or monocytes loaded with whole insulin (INS) or insulin β chain peptide 9-23 (B:9-23). (C) Graphs presenting changes in cells populations. Only modifications in which statistical significance occurs are presented. Cells responding to the antigen have an index SPEC, cells not recognizing the antigen have an index UNSPEC. All analyzes were performed for six tests (three tests for whole insulin and three tests for insulin β chain peptide 9-23) in triplicate. The results are presented as mean+/- SD. Significance was calculated using the t- test, significant results are marked with * (p<0.05), ** (p<0.01) or *** (p<0.001). Heat maps were prepared using ClustVis tool based on correlation distance and average linkage between clusters (21). ↑ - activating modification; ↓ - inactivating modification; ··­·· difference between specific Tregs and Teffs stimulated with insulin; ···· difference between unspecific Tregs and Teffs stimulated with insulin; ··­·· ­­­ difference between unspecific Tregs and Teffs stimulated with peptide 9-23.