Table 1.
Sequences of primers used for real-time PCR.
| Gene Name | RefSeq Accession Number | Primer | Amplicon Size (nt) | %GC | Tm | Primer Sequence (5’-3’) Forward | Probe # |
|---|---|---|---|---|---|---|---|
| GAPDH* | NM_001289745.1 | Fw | 70 | 45 | 60 | CCCCGGTTTCTATAAATTGAGC | #75 |
| Rv | 58 | 59 | GGCTGACTGTCGAACAGGA | ||||
| FoxP3 | NM_014009.3 | Fw | 102 | 55 | 59 | ACACTGCCCCTAGTCATGGT | #25 |
| Rv | 50 | 60 | GAGCTGGTGCATGAAATGTG | ||||
| CTLA-4 | NM_005214.4 | Fw Rv | 65 | 56 50 | 60 60 | TGGGTCCCAGGGAAGTTT TGACCTTGTGTTCTACCTGGTG | #25 |
| IKZF2 | NM_016260.2 | Fw | 64 | 45 | 59 | CATCACATTGCTTTGCCCTA | #61 |
| Rv | 48 | 59 | TCATCACTGTCAGAGAGAGGCTA | ||||
| IKZF4 | NM_022465.3 | Fw | 68 | 45 | 60 | TCAGGCATTTGTTGTGCAGT | #3 |
| Rv | 53 | 59 | AGGGAAAGGCAGATGCTGT | ||||
| IL2RA | NM_000417.2 | Fw | 73 | 55 | 59 | CCAACTTCCCAGTTCAGGAG | #45 |
| Rv | 44 | 59 | GGGTAGAGTGTGTGTGTTGTGTATT | ||||
| TNFRSF18 | NM_004195.2 | Fw | 92 | 61 | 59 | ACCTGGGTCGGGATTCTC | #10 |
| Rv | 61 | 59 | CACAGCCAGTTGGACACG | ||||
| Tet2 | NM_001127208.2 | Fw | 93 | 36 | 59 | AAAGATGAAGGTCCTTTTTATACCC | #68 |
| Rv | 48 | 59 | ACCCTTCTGTCCAAACCTTTC | ||||
| Runx1 | NM_001754.4 | Fw | 61 | 41 | 60 | CCAAAGAGTGTGGAATTTTGGT | #55 |
| Rv | 50 | 59 | AAACAGGGCGAGTTGCAT | ||||
| HMOX1 | NM_002133.2 | Fw | 61 | 55 | 59 | CCCTTCAGCATCCTCAGTTC | #84 |
| Rv | 58 | 59 | GACAGCTGCCACATTAGGG |
*GAPDH was used as an endogenous control.