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Primers for TSDR analysis (19).
| Primer set | Primer | Primer Sequence (5’-3’) Forward | PCR product size | Tm |
|---|---|---|---|---|
| demethylated | Fw Rv | GGATAGGGTAGTTAGTTTTTGGAATG CCACCATTAACATCATAACAACCA | 117 | 62.6 64.1 |
| methylated | Fw Rv | GATAGGGTAGTTAGTTTTCGGAAC CCGCCATTAACGTCATAACG | 116 | 59.9 64.9 |
