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. 2021 Mar 31;15:643067. doi: 10.3389/fnana.2021.643067

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Copyright © 2021 Yamashiro, Liu, Matsumoto and Ikegaya.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Differences in the immunofluorescence and shape of GAD67-positive and GAD67-negative cells in the primary somatosensory and motor cortices. (A) Histogram of the fluorescence of anti-GAD67 signals of manually annotated GAD67-positive (red) and -negative (gray) neurons in S1. (B) The same as (A), but for anti-NeuN immunofluorescence signals. (C) The same as (A), but for Nissl fluorescence signals. (D) The same as (A), but for the area of individual neurons (i.e., ROIs). (E) The same as (A), but for the circularity of individual neurons (i.e., ROIs). (F–J) The same as (A–E), respectively, but for M1. Abbreviations: S1, primary somatosensory cortex; M1, primary motor cortex; ROI, region of interest.