Figure 1.

Four-step modular approach for high dimensional OMICS analysis for immune profiling during disease and (immuno)-therapy. Steps consist of (1) sample preparation, (2) cell barcoding and staining, (3) sample acquisition, and (4) data analysis. Briefly, single cells or histologic slides are prepared from blood, or fixed tissue samples. Single cells from blood or dissociated fresh tissues are barcoded, stained for live/dead, surface and/or intracellular markers and acquired using single cell solution mass cytometry. As an alternative CiteSeq can be utilized. Tissues on histologic slides are stained and acquired using imaging mass cytometry. For mass cytometry, data is bead-normalized and randomized. After de-barcoding data can be loaded in the bioinformatic analysis platform of choice.